Intermediate

Part:BBa_K1613023:Design

Designed by: Han, Yun-An   Group: iGEM15_HSNU-TAIPEI   (2015-09-14)

B0015:I721001


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 217

This part is a ligation of B0015 and I721001---the former fragments of K1613017. The description below is from the information of K1613017. This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.

K1613023-1.jpg

Design Notes

At first, we used I721001 and I721002 from 2012 kit plate, but then we found out that they were inconsistent because of the failed transformation we did. So we got the sequences of these two parts from genebank and ordered these two parts.


Source

The sequence was retrieved from genebank.

References